Physical Chemistry: Principles and Applications in Biological Sciences (5e) : 9780136056065

Physical Chemistry: Principles and Applications in Biological Sciences (5e)

Tinoco, Ignacio et al
Published by
Pearson Higher Ed USA
Available on demand
Title type
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Written specifically for the life-science student, the 5th Edition of the bestselling Physical Chemistry: Principles and Applications in Biological Sciences covers core aspects of biophysical chemistry–while showing how biochemists and biophysicists use principles of physical vhemistry to solve real problems in biological systems.


Table of contents
  • Chapter 1: Introduction
  • Chapter 2: The First Law: Energy is Conserved
  • Chapter 3: The Second Law: The Entropy of the Universe Increases
  • Chapter 4: Free Energy and Chemical Equilibria
  • Chapter 5: The Statistical Foundations of Biophysical Chemistry
  • Chapter 6: Physical Equilibria
  • Chapter 7: Electrochemistry
  • Chapter 8:  The Motion of Biological Molecules
  • Chapter 9: Kinetics: Rates of Chemical Reactions
  • Chapter 10: Enzyme Kinetics
  • Chapter 11: Molecular Structures and Interactions: Theory
  • Chapter 12: Molecular Structures and Interactions: Biomolecules
  • Chapter 13: Optical Spectroscopy
  • Chapter 14: Magnetic Resonance
  • Chapter 15: Macromolecular Structure and X-Ray Diffraction
New to this edition
  • NEW sequence of chapters: Content has been expanded, updated, and reorganized to aid instructors’ coverage and discussion of important topics within physical chemistry. Theoretical concepts now appear up front sooner and with more breadth for statistical methods and includes a new chapter devoted to Electrochemistry (Chapter 7). Comprehension of the fundamental principles is key to a successful life-science career.
  • NEW visual program with over 200 pieces of new and revised figures helps students visualize and understand the concepts discussed within each chapter.
  • Updated to current IUPAC conventions for relevancy.
  • A NEW appendix (Math Appendix A) in the Fifth Edition provides an accessible, concise, and self-contained review of the mathematics that students are expected to know in order to succeed in this course. Emphasizing the practical applications of a core set of mathematical methods, this appendix also includes an introduction to vector spaces in quantum mechanics.
  • Coverage of molecular spectroscopy has been expanded into two more focused chapters, with more grounding in underlying principles that students will need to master in order to pursue a life science career. The chapter on optical spectroscopy (chapter 13) gives expanded presentations of fluorescence and IR spectroscopies of proteins, while the magnetic resonance chapter (chapter 14) now includes multi-dimensional and diffusion NMR and their applications to biomolecules.
  • Specific examples of statistical weights in ligand binding and molecular conformations help students understand hormone and drug binding to receptors, and the folding of proteins and nucleic acids.
  • Updated applications engage students by introducing them to the latest research applications of physical chemistry important in biochemistry and molecular biology.
  • Coverage of quantum chemistry has been expanded into two chapters (11 and 12) with elaboration and updates on the contemporary work in this field and applications in biophysics
Features & benefits
  • A streamlined treatment covers the core aspects of biophysical chemistry (thermodynamics and kinetics as well as quantum mechanics, spectroscopy, and X-ray diffraction), which are of great importance to students of biology and biochemistry. Essentially all applications of the concepts are of interest to life-science students; nearly all the problems apply to life-science examples.
  • Clear, explicit writing helps students, especially those with weaker math skills, cement their conceptual understanding of physical chemistry
  • An appropriate level of mathematics uses elementary calculus to derive equations and carefully defines the scope of mathematics needed to solve problems.
  • Emphasis on methods such as modern fluorescence, calorimetry and single molecule techniques introduces students to techniques that are important in biochemistry and molecular biology
  • Theory of molecular structure and interactions begins with the origins of quantum mechanics, and builds up to intermolecular and intramolecular forces and the use of semi-empirical methods to calculate molecular conformation, giving students a better understanding of the concepts and methods used to calculate and predict macromolecular structures
  • Emphasis on nuclear magnetic resonance and fluorescence spectroscopy provides an in-depth look at the two main methods used to study proteins and nucleic acids in solution.
  • Updated coverage of molecular structures and X-ray diffraction (Ch. 15) offers a detailed discussion of how a structure is obtained from a measured diffraction pattern. X-ray diffraction available to non-specialists to determine structures of proteins and nucleic acids.
Author biography

Ignacio Tinoco was an undergraduate at the University of New Mexico, a graduate student at the University of Wisconsin, and a postdoctoral fellow at Yale. He then went to the University of California, Berkeley, where he has remained. His research interest has been on the structures of nucleic acids, particularly RNA. He was chairman of the Department of Energy committee that recommended in 1987 a major initiative to sequence the human genome. His present research is on unfolding single RNA molecules by force.


Kenneth Sauer grew up in Cleveland, Ohio, and received his A.B. in chemistry from Oberlin College. Following his Ph.D. studies in gas-phase physical chemistry at Harvard, he spent three years teaching at the American University of Beirut, Lebanon. A postdoctoral opportunity to learn from Melvin Calvin about photosynthesis in plants led him to the University of California, Berkeley, where he has been since 1960. Teaching general chemistry and biophysical chemistry in the Chemistry Department has complemented research in the Physical Biosciences Division of the Lawrence Berkeley National Lab involving spectroscopic studies of photosynthetic light reactions and their role in water oxidation. His other activities include reading, renaissance and baroque choral music, canoeing, and exploring the Sierra Nevada with his family and friends.

James C. Wang was on the faculty of the University of California, Berkeley, from 1966 to 1977. He then joined the faculty of Harvard University, where he is presently Mallinckrodt Professor of Biochemistry and Molecular Biology. His research focuses on DNA and enzymes that act on DNA, especially a class of enzymes known as DNA topoisomerases. He has taught courses in biophysical chemistry and molecular biology and has published over 200 research articles. He is a member of Academia Sinica, the American Academy of Arts and Sciences, and the U.S. National Academy of Sciences.


Joseph Puglisi was born and raised in New Jersey. He received his B.A. in chemistry from The Johns Hopkins University in 1984 and his Ph.D. from the University of California, Berkeley, in 1989. He has studied and taught in Strasbourg, Boston, and Santa Cruz, and is currently professor of structural biology at Stanford University. His research interests are in the structure and mechanism of the ribosome and the use of NMR spectroscopy to study RNA structure. He has been a Dreyfus Scholar, Sloan Scholar, and Packard Fellow.

Gerard Harbison was born in the United Kingdom and raised there and in Ireland. He received his B.A. in biochemistry from Trinity College, Dublin, and his Ph.D. in biophysics from Harvard University. After a brief postdoctoral sojourn at the Max-Planck Institute for Polymer Research in Mainz, Germany, he joined the faculty of Stony Brook University, and then moved to the University of Nebraska Lincoln. He is a Dreyfus Scholar, Lilly Foundation Teacher-Scholar and Presidential Young Investigator. His research interests are in nuclear magnetic resonance and electronic structure theory.


David Rovnyak, a native of Charlottesville, Virginia, earned his B.S. in Chemistry at the University of Richmond and Ph.D. in physical chemistry from the Massachusetts Institute of Technology.  After performing post-doctoral study at the Harvard Medical School under an NIH-NRSA fellowship, he joined Bucknell University where he has been recognized with the Bucknell Presidential Teaching Award for Excellence.  His research focuses on new methods for NMR spectroscopy and physico-chemical behavior of bile acids.

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